CovidV2, Main, Exploration, bibRecord, 001688

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

Identifieur interne : 001688 ( Main/Exploration ); précédent : 001687; suivant : 001689

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

Auteurs : N. Zari [Maroc] ; H. Mohammedi [Maroc] ; A. Amine [Maroc] ; M. M. Ennaji [Maroc]

Source :

Analytical letters [ 0003-2719 ] ; 2007.

RBID : Pascal:07-0341588

Descripteurs français

Pascal (Inist)
- Hydrolyse acide, Voltammétrie, Biodétecteur, Onde carrée, Méthode électrochimique, Oxydation, Electrode carbone, Electrode pâte, Sensibilité, Analyse trace, Température ambiante, Capteur chimique, DNA, Guanine, Adénine, Or.
Wicri :
- topic : Or.

English descriptors

KwdEn :
- Acid hydrolysis, Adenine, Biosensor, Carbon electrode, Chemical sensor, DNA, Electrochemical method, Gold, Guanine, Oxidation, Paste electrode, Room temperature, Sensitivity, Square wave, Trace analysis, Voltammetry.

Abstract

This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.

Affiliations:

Maroc

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000034
to stream PascalFrancis, to step Curation: 000025
to stream PascalFrancis, to step Checkpoint: 000036
to stream Main, to step Merge: 001694
to stream Main, to step Curation: 001688

Le document en format XML

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<term>Chemical sensor</term>
<term>DNA</term>
<term>Electrochemical method</term>
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<term>Guanine</term>
<term>Oxidation</term>
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<term>Room temperature</term>
<term>Sensitivity</term>
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<term>Trace analysis</term>
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<term>Méthode électrochimique</term>
<term>Oxydation</term>
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<term>Electrode pâte</term>
<term>Sensibilité</term>
<term>Analyse trace</term>
<term>Température ambiante</term>
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<front><div type="abstract" xml:lang="en">This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and  Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.</div>
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DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri